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Equipment List

Below is a list of the major items of equipment available for use at the OCCM. Reading the descriptions will help you decide which is most suitable for your needs. Which doesn't mean you can then just go ahead and use them without first registering your project and learning how to use them.

If you have any further questions on using any of the equipment, please contact Andrew McNaughton at andrew.mcnaughton@otago.ac.nz.
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Zeiss LSM 710 Confocal Microscope

Upright configuration on an Axio Imager Z2 frame with 405 diode laser, argon laser, green HeNe and red HeNe laser, phase objectives and mercury burner. Laser excitation lines are: 405nm 458nm, 488nm, 514nm, 543nm and 633nm
This configuration is best suited for fixed material on standard microscope slides. It can unmix overlapping spectra that are difficult to separate on the Zeiss 510 confocals. It can also perform tile scans over the Z axis which is useful for large samples. For many samples, and for student work, it is recommended to use one of the Zeiss 510 systems (see below) as they are cheaper and ideal for straight forward protocols.
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Zeiss LSM 510 Upright Confocal Microscope

Upright configuration on an Axioplan 2 frame with argon laser, green HeNe and red HeNe laser, phase objectives and mercury burner. Laser excitation lines are: 458nm, 477nm, 488nm, 514nm, 543nm and 633nm
This configuration is best suited to fixed material on standard microscope slides. For live cell work you are best to use the inverted system.
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Zeiss LSM 510 Inverted Confocal Microscope

Inverted configuration on an Axiovert 200 frame with argon laser, green HeNe and red HeNe laser, phase objectives and mercury burner. Laser excitation lines are: 458nm, 477nm, 488nm, 514nm, 543nm and 633nm.
Best suited for live cell work, the system can accommodate a variety of cell plates, petri dishes and standard microscope slides. Note, if you are using well plates with non-optical quality bases you will be limited to using the x4 and x10 objectives. For quality images you require bases designed for viewing with a compound microscope.
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Olympus AX70 Light Microscope

A standard research level light microscope with brightfield, phase contrast, dark field and fluoescence, the AX70 has an upright configuration and is suited to fixed material on standard microscope slides.
Fluorescence ranges from uv, blue, green and red excitation using a xenon burner. Epi-fluorescence is also available and is useful for such techniques as silver enhanced immuno-gold labelling.
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StereoInvestigator

A sophisticated stereology software package integrated onto an Olympus BX51 light microscope with motorised xy stage and z stepper motor. This system is capable of complex stereological analysis of your light microscopy images, including those collected on other light and the confocal microscopes. Read More …
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Skyscan µCT Scanner

A high resolution µCT scanner, the Skyscan 1172 uses x-ray tomography to generate 3D data sets of specimens measuring up to 55x55x55mm. Resolutions of 2µm are possible and a wide range of specimens can be examined, from food to teeth. Soft tissues usually require a contrasting agent to be used. Large amounts of data (4-8 GB per sample) are produced and if volume rendering is required, a large amount of computing capacity too. Ask about the best plan of attack while planning an investigation.