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Replacement Confocal Microscope: Nikon A1+ TiE Inverted


After many years of faithful service, one of our upright confocals, the Zeiss LSM 510 Axioplan, is being retired and re-purposed as a fluorescence microscope. It’s replacement is a Nikon A1+ inverted confocal, suitable for slide mounted specimens (by turning your slide over), but also able to accommodate well-plates, small petri dishes and other specialised culture cells.

This confocal is now commissioned and open for bookings, following appropriate training. Please do not attempt to use it without training, because ‘it’s just like the other confocal’ or you think ‘winging it’ might count as research - it doesn’t. If you break something because of inappropriate use, you may end up paying for it - and make your colleagues very angry - let’s not forget that aspect.

The purchase is a joint venture between the departments of Anatomy and Physiology, it will also be available for use by other departments, at a higher hourly rate, similar to that charged for use of the Zeiss LSM 710 confocal microscope.

Please contact Andrew McNaughton for further details and enquires about training and subsequent bookings.
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Specifications


Nikon Ti-E, Motorised Inverted Microscope Motorised XY stage
Motorised 6 position nose piece Fluorescence illumination and standard filters Nikon Lambda Objectives (10x,20x,40x,60x)
A1+ Confocal Scan Head
Transmitted light detector
4 component high-sensitivity GaAsP detector unit
16MP DS-Ri2 colour camera
LU-N4S laser unit (405nm, 488nm, 561nm, 640nm excitation lines)
NIS Elements C-ER super-resolution package NIS-Elements JOBS and General Analysis

Confocal: How it Works

For most biological samples, a fluorescent marker(s) is required to use the confocal microscope.

Generally, if you've successfully stained your samples and viewed the results under an ordinary epi-fluorescence microscope, your samples will be suitable for the confocal. In other words, you've already done 90% of what's required.

Light Microscopy

We have a number of research grade light microscopes available for use. Most also have epi-fluorescence as well as bright field capabilities.

Other, more specialised microscopes, can obtain montages of large specimens or help you with stereology investigations.

ImageJ and Analysis

Open source software. like ImageJ, can provide an excellent platform to base image analysis upon. There's a lot more to simply obtaining a 'pretty picture' with any of the equipment listed here.

You should be able to at least make semi-quantitative assessments of the data you have collected. This is where image analysis come in.

µCT: Sample Suitability

The µCT uses Computerised Axial Tomography (CAT) to produce a series of digital slices of solid objects. Best suited to relatively dense material like teeth and bone, the µCT can resolve details down to about 1µm.
If you're looking for electron microscopy techniques, go here: OCEM

Contact Details

Andrew McNaughton
03 479 7308
andrew.mcnaughton@otago.ac.nz

Room B01g Basement
Department of Anatomy
Lindo Ferguson Building
270 Great King Street
(Opposite main entrance to Public Hospital, Great King Street)
PO Box 913
Dunedin 9001
New Zealand